An <em>In Vitro</em> Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
نویسندگان
چکیده
منابع مشابه
Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDermTM
Effects of nanoparticles (NPs) on skin corrosion and irritation using three-dimensional human skin models were investigated based on the test guidelines of Organization for Economic Co-operation and Development (OECD TG431 and TG439). EpiDermTM skin was incubated with NPs including those harboring iron (FeNPs), aluminum oxide (AlNPs), titanium oxide (TNPs), and silver (AgNPs) for a defined time...
متن کاملSkin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model
OBJECTIVES Effects of nanoparticles including zinc oxide nanoparticles, titanium oxide nanoparticles, and their mixtures on skin corrosion and irritation were investigated by using in vitro 3D human skin models (KeraSkin ((TM)) ) and the results were compared to those of an in vivo animal test. METHODS Skin models were incubated with nanoparticles for a definite time period and cell viability...
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INTRODUCTION 1. Skin irritation refers to the production of reversible damage to the skin following the application of a test chemical for up to 4 hours [as defined by the United Nations (UN) Globally Harmonized System of Classification and Labelling of Chemicals (GHS)](1). This Test Guideline provides an in vitro procedure that may be used for the hazard identification of irritant chemicals (s...
متن کاملDose-response Evaluation Using an Epidermal Model, an Alternative to Skin Irritation Testing
Using 3-dimensional cultured human skin and epidermal models, an alternative to skin irritation testing have been developed. In the ECVAM (European Centre for the Validation of Alternative Methods) validation protocol, hazardous chemicals have been identified using these models. We considered it likely that these models would also be useful for examining the dose-response of the toxicity of a c...
متن کاملReview Articles in Vitro Safety Testing Strategy for Skin Irritation Using the 3d Reconstructed Human Epidermis
One of the most recent trends in skin irritation safety testing of personal care and cosmetic products, discussed in detail in this review, is the use of reconstructed human epidermis models. Some of the commercially-available models have undergone a series of validation trials, which successfully advanced them as valuable tools for industry use. On a broader perspective, the 3D models are now ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2009
ISSN: 1940-087X
DOI: 10.3791/1366